Journal: Cell reports
Article Title: Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo
doi: 10.1016/j.celrep.2021.109227
Figure Lengend Snippet: (A) Flow cytometry analysis of CD27 expression by γδ T cells in lymph nodes and lung of fetal-, neonatal-, or adult-induced RBPJ ind mice (left); right panels show percentages. (B) Flow cytometry analysis of IL-17 production by γδ T cells in lymph nodes and lung of mice as in (A) stimulated with PMA and ionomycin in vitro (left); right panels show percentages. Data are representative of three independent experiments. Data are presented as means ± standard deviation of three independent experiments. n = 3 mice per group. **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA). (C) Flow cytometry analysis of IL-17 production by γδ T cells in lung of control, fetal-, or adult-induced RBPJ ind mice after TDM challenge (top); bottom panels show percentages and numbers. Data are representative of three independent experiments. Data are presented as means ± standard deviation of three independent experiments. n = 6 mice for control; n = 3 mice per fetal- and adult-induced groups. Statistical analyses between groups are found in .
Article Snippet: APC-Cy7 anti-mouse CD27 , eBiosciences , 47027182.
Techniques: Flow Cytometry, Expressing, In Vitro, Standard Deviation, Control